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Validation Data Gallery

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  • Western Blot (WB) analysis of Karpas-299 cells using CD30/TNFRSF8 Recombinant antibody (84241-2-RR)

    WB analysis of Karpas-299 using 84241-2-RR

    Karpas-299 cells were subjected to SDS PAGE followed by western blot with 84241-2-RR (CD30/TNFRSF8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84241-2-RR

    Affinity and Kinetic Characterization of 84241-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84241-2-RR against Human CD30/TNFRSF8 were performed. The affinity constant is 0.391 nM.

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CD30/TNFRSF8 Recombinant antibody

CD30/TNFRSF8 Recombinant Antibody for WB, ELISA
Cat No. 84241-2-RR
Clone No.241330G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD30, 241330G11, Ki 1 antigen, Ki 1, D1S166E

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inKarpas-299 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84241-2-RR targets CD30/TNFRSF8 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 8
Calculated molecular weight 64 kDa
Observed molecular weight120 kDa
GenBank accession numberNM_001243
Gene Symbol CD30
Gene ID (NCBI) 943
RRIDAB_3671793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28908
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).

Protocols

Product Specific Protocols
WB protocol for CD30/TNFRSF8 antibody 84241-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols