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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD30/TNFRSF8 Recombinant antibody, PBS Only (Capture)

CD30/TNFRSF8 Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241330G11

Cat no : 84241-2-PBS

Synonyms

CD30, 241330G11, Ki 1 antigen, Ki 1, D1S166E

Validation Data Gallery

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  • Western Blot (WB) analysis of Karpas-299 cells using CD30/TNFRSF8 Recombinant antibody (84241-2-RR)

    WB analysis of Karpas-299 using 84241-2-RR (same clone as 84241-2-PBS)

    Karpas-299 cells were subjected to SDS PAGE followed by western blot with 84241-2-RR (CD30/TNFRSF8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84241-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01137-1

    Sandwich ELISA standard curve of MP01137-1

    Sandwich ELISA standard curve of MP01137-1, Human CD30/TNFRSF8 Recombinant Matched Antibody Pair - PBS only. 84241-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02334. 84241-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-1250 pg/mL.

  • Sample test of MP01137-1

    Sample test of MP01137-1

    Serum of six individual healthy human donors was measured. The CD30/TNFRSF8 concentration of detected samples was determined to be 1,639.1 pg/mL with a range of 1,021.2-2,597.7 pg/mL.

  • Cytometric bead array standard curve of MP01137-1

    Cytometric bead array standard curve of MP01137-1

    Cytometric bead array standard curve of MP01137-1, TNFRSF8/CD30 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84241-2-PBS. Detection antibody: 84241-4-PBS. Standard: RP02334. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01137-2

    Cytometric bead array standard curve of MP01137-2

    Cytometric bead array standard curve of MP01137-2, TNFRSF8/CD30 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84241-2-PBS. Detection antibody: 84241-3-PBS. Standard: RP02334. Range: 0.625-40 ng/mL.

  • Cytometric bead array standard curve of MP01137-3

    Cytometric bead array standard curve of MP01137-3

    Cytometric bead array standard curve of MP01137-3, TNFRSF8/CD30 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84241-2-PBS. Detection antibody: 84241-1-PBS. Standard: RP02334. Range: 0.313-20 ng/mL.

  • Affinity and Kinetic Characterization of 84241-2-RR

    Affinity and Kinetic Characterization of 84241-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84241-2-RR against Human CD30/TNFRSF8 were performed. The affinity constant is 0.391 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84241-2-PBS targets CD30/TNFRSF8 as part of a matched antibody pair:

MP01137-1: 84241-2-PBS capture and 84241-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01137-2: 84241-2-PBS capture and 84241-3-PBS detection (validated in Cytometric bead array)

MP01137-3: 84241-2-PBS capture and 84241-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 8
Calculated molecular weight 64 kDa
Observed molecular weight120 kDa
GenBank accession numberNM_001243
Gene symbol CD30
Gene ID (NCBI) 943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).