CD31 Monoclonal antibody, PBS Only (Capture)
CD31 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
3F8E2
Cat no : 66065-2-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
66065-2-PBS targets CD31 as part of a matched antibody pair:
MP50319-2: 66065-2-PBS capture and 66065-5-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD31 fusion protein Ag19730 相同性解析による交差性が予測される生物種 |
Full Name | platelet/endothelial cell adhesion molecule |
Calculated molecular weight | 83 kDa |
Observed molecular weight | 120-130 kDa |
GenBank accession number | BC022512 |
Gene symbol | CD31 |
Gene ID (NCBI) | 5175 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).