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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD31 Monoclonal antibody, PBS Only (Capture)

CD31 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F8E2

Cat no : 66065-2-PBS

Synonyms

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD31 Monoclonal antibody (66065-2-Ig)

    WB analysis using 66065-2-Ig (same clone as 66065-2-PBS)

    Untreated and PNGase F-treated lysates of human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using CD31 Monoclonal antibody (66065-2-Ig)

    WB analysis of human peripheral blood platelets using 66065-2-Ig (same clone as 66065-2-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD31 Monoclonal antibody (66065-2-Ig)

    WB analysis using 66065-2-Ig (same clone as 66065-2-PBS)

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CD31 Monoclonal antibody (66065-2-Ig)

    WB analysis of Jurkat using 66065-2-Ig (same clone as 66065-2-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using CD31 Monoclonal antibody (66065-2-Ig)

    WB analysis of THP-1 using 66065-2-Ig (same clone as 66065-2-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD31 Monoclonal antibody (66065-2-Ig)

    IHC staining of human liver cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD31 Monoclonal antibody (66065-2-Ig)

    IHC staining of human liver cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD31 Monoclonal antibody (66065-2-Ig)

    IHC staining of human tonsillitis using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD31 Monoclonal antibody (66065-2-Ig)

    IHC staining of human tonsillitis using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD31 Monoclonal antibody (66065-2-Ig)

    IHC staining of human breast cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD31 Monoclonal antibody (66065-2-Ig)

    IHC staining of human breast cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CD31 Monoclonal antibody (66065-2-Ig)

    IF Staining of human liver cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CD31 Monoclonal antibody (66065-2-Ig)

    IF Staining of human liver cancer using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CD31 Monoclonal antibody (66065-2-Ig)

    IF Staining of human placenta using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CD31 Monoclonal antibody (66065-2-Ig)

    IF Staining of human placenta using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CD31 Monoclonal antibody (66065-2-Ig)

    IF Staining of HUVEC using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CD31 Monoclonal antibody (66065-2-Ig)

    IF Staining of HUVEC using 66065-2-Ig (same clone as 66065-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), Beta Tubulin antibody (80713-1-RR, Clone: 2O13, red). This data was developed using the same antibody clone with 66065-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50319-2

    Cytometric bead array standard curve of MP50319-2

    Cytometric bead array standard curve of MP50319-2, CD31 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66065-2-PBS. Detection antibody: 66065-5-PBS. Standard:Ag1787. Range: 3.125-200 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66065-2-PBS targets CD31 as part of a matched antibody pair:

MP50319-2: 66065-2-PBS capture and 66065-5-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD31 fusion protein Ag19730 相同性解析による交差性が予測される生物種
Full Name platelet/endothelial cell adhesion molecule
Calculated molecular weight 83 kDa
Observed molecular weight120-130 kDa
GenBank accession numberBC022512
Gene symbol CD31
Gene ID (NCBI) 5175
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).