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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CD31 Recombinant antibody

CD31 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4N13

Cat no : CL488-80530

Synonyms

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite® Plus 488-conjugated CD31 Recombinant ant (CL488-80530)

    IF Staining of HUVEC using CL488-80530

    Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using CoraLite® Plus 488 CD31 antibody (CL488-80530, Clone: 4N13 ) at dilution of 1:100, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite® Plus 488-conjugated CD31 Recombinant ant (CL488-80530)

    IF Staining of HUVEC using CL488-80530

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 CD31 antibody (CL488-80530, Clone: 4N13 ) at dilution of 1:100, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Tested Applications

Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80530 targets CD31 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD31 fusion protein Ag1787 相同性解析による交差性が予測される生物種
Full Name platelet/endothelial cell adhesion molecule
Calculated molecular weight 83 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC022512
Gene symbol CD31
Gene ID (NCBI) 5175
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD31 antibody CL488-80530Download protocol
Standard Protocols
Click here to view our Standard Protocols