CoraLite®594-conjugated CD31 Monoclonal antibody
CD31 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3F8E2
Cat no : CL594-66065
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human tonsillitis tissue, human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
CL594-66065 targets CD31 in WB, IF-P applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD31 fusion protein Ag1787 相同性解析による交差性が予測される生物種 |
| Full Name | platelet/endothelial cell adhesion molecule |
| Calculated molecular weight | 83 kDa |
| GenBank accession number | BC022512 |
| Gene symbol | CD31 |
| Gene ID (NCBI) | 5175 |
| RRID | AB_2883500 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).This antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CD31 antibody CL594-66065 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity |
