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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FCGR2A / CD32a Monoclonal antibody

FCGR2A / CD32a Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D3F1

Cat no : 66529-1-Ig

Synonyms

CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FCGR2A / CD32a Monoclonal antibody (66529-1-Ig)

    WB analysis using 66529-1-Ig

    Untreated and PNGase F-treated lysates of THP-1 cells and Mo7e cells were subjected to SDS PAGE followed by western blot with 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using FCGR2A / CD32a Monoclonal antibody (66529-1-Ig)

    WB analysis of U-937 using 66529-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using FCGR2A / CD32a Monoclonal antibody (66529-1-Ig)

    WB analysis of Raji using 66529-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FCGR2A / CD32a Monoclonal antibody (66529-1-Ig)

    IHC staining of human liver cancer using 66529-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FCGR2A / CD32a Monoclonal antibody (66529-1-Ig)

    IHC staining of human liver cancer using 66529-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FCGR2A / CD32a Monoclonal antibody (66529-1-Ig)

    IHC staining of human tonsillitis using 66529-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FCGR2A / CD32a Monoclonal antibody (66529-1-Ig)

    IHC staining of human tonsillitis using 66529-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using FCGR2A / CD32a Monoclonal antibody (66529-1-Ig)

    IF Staining of THP-1 using 66529-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FCGR2A / CD32a antibody (66529-1-Ig, Clone: 1D3F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inTF-1 cells, Raji cells, U-937 cells, Mo7e cells, K-562 cells, THP-1 cells
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66529-1-Ig targets FCGR2A / CD32a in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FCGR2A / CD32a fusion protein Ag25878 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Calculated molecular weight 35 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC020823
Gene symbol CD32a
Gene ID (NCBI) 2212
RRIDAB_2881892
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

Protocols

Product Specific Protocols
WB protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
IHC protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
IF protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols