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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated FCGR2A / CD32a Monoclonal antibody

FCGR2A / CD32a Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D3F1

Cat no : CL488-66529

Synonyms

CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A / CD32a, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated FCGR2A / CD32a Monoc (CL488-66529)

    IF Staining of human tonsillitis using CL488-66529

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66529 (FCGR2A / CD32a antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated FCGR2A / CD32a Monoc (CL488-66529)

    IF Staining of human tonsillitis using CL488-66529

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66529 (FCGR2A / CD32a antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CoraLite® Plus 488-conjugated FCGR2A / CD32a Monoc (CL488-66529)

    IF Staining of THP-1 using CL488-66529

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 FCGR2A / CD32a antibody (CL488-66529, Clone: 1D3F1 ) at dilution of 1:50.

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inTHP-1 cells, human tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66529 targets FCGR2A / CD32a in IF/ICC, IF-P applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FCGR2A / CD32a fusion protein Ag25878 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Calculated molecular weight 35 kDa
GenBank accession numberBC020823
Gene symbol CD32a
Gene ID (NCBI) 2212
RRIDAB_2883350
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FCGR2A / CD32a antibody CL488-66529Download protocol
Standard Protocols
Click here to view our Standard Protocols