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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR)

    FC experiment of human PBMCs using 98184-1-RR (same clone as 98184-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98184-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR)

    FC experiment of human PBMCs using 98184-1-RR (same clone as 98184-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98184-1-PBS in a different storage buffer formulation.

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Anti-Human CD33 Rabbit Recombinant Antibody, PBS Only

CD33 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98184-1-PBS
Clone No.241711C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SIGLEC 3, Sialic acid-binding Ig-like lectin 3, p67, gp67, CD33 molecule

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98184-1-PBS targets CD33 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name CD33 molecule
Calculated molecular weight 364 aa, 40 kDa
GenBank accession numberBC028152
Gene Symbol CD33
Gene ID (NCBI) 945
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP20138
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.