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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD33 Rabbit Recombinant Antibody

CD33 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241711C12

Cat no : 98184-1-RR

Synonyms

SIGLEC 3, Sialic acid-binding Ig-like lectin 3, p67, gp67, CD33 molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR)

    FC experiment of human PBMCs using 98184-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR)

    FC experiment of human PBMCs using 98184-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98184-1-RR targets CD33 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name CD33 molecule
Calculated molecular weight 364 aa, 40 kDa
GenBank accession numberBC028152
Gene symbol CD33
Gene ID (NCBI) 945
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.

Protocols

Product Specific Protocols
FC protocol for CD33 antibody 98184-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols