CoraLite®594-conjugated CD34 Monoclonal antibody
CD34 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
4F11H3
Cat no : CL594-60180-2
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-60180-2 targets CD34 in IF-P applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD34 fusion protein Ag5996 相同性解析による交差性が予測される生物種 |
| Full Name | CD34 molecule |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 105 kDa |
| GenBank accession number | BC039146 |
| Gene symbol | CD34 |
| Gene ID (NCBI) | 947 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CD34 antibody CL594-60180-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
