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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated CD34 Monoclonal antibody

CD34 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C8G12

Cat no : CL594-60180

Synonyms

CD34, CD34 molecule

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite®594-conjugated CD34 Monoclonal antibody (CL594-60180)

    IF Staining of human liver cancer using CL594-60180

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 CD34 antibody (CL594-60180, Clone: 3C8G12 ) at dilution of 1:100, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta), CoraLite® Plus 488 CD163 antibody (CL488-16646, green).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CoraLite®594-conjugated CD34 Monoclonal antibody (CL594-60180)

    IF Staining of human placenta using CL594-60180

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite®594 CD34 antibody (CL594-60180, Clone: 3C8G12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CoraLite®594-conjugated CD34 Monoclonal antibody (CL594-60180)

    IF Staining of human placenta using CL594-60180

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite®594 CD34 antibody (CL594-60180, Clone: 3C8G12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD34 Monoclonal antibody (CL594-60180)

    IF Staining of human tonsillitis using CL594-60180

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite®594-conjugated version of this antibody, CL594-60180 (CD34 antibody), at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD34 Monoclonal antibody (CL594-60180)

    IF Staining of human tonsillitis using CL594-60180

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite®594-conjugated version of this antibody, CL594-60180 (CD34 antibody), at dilution of 1:100.

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Tested Applications

Positive IF-P detected inhuman liver cancer tissue, human tonsillitis tissue, human placenta tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60180 targets CD34 in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD34 fusion protein Ag5996 相同性解析による交差性が予測される生物種
Full Name CD34 molecule
Calculated molecular weight 41 kDa
GenBank accession numberBC039146
Gene symbol CD34
Gene ID (NCBI) 947
RRIDAB_2883435
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Protocols

Product Specific Protocols
IF protocol for CL594 CD34 antibody CL594-60180Download protocol
Standard Protocols
Click here to view our Standard Protocols