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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 594 Anti-Human CD35 (E11)

CD35 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

E11

Cat no : CL594-65254

Synonyms

KN, E11, CR1, Complement receptor type 1, C3BR

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 594 Anti-Human CD35 (E11) (CL594-65254)

    FC experiment of human PBMCs using CL594-65254

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD35 (CL594-65254, Clone:E11), or CoraLite®594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 594 Anti-Human CD35 (E11) (CL594-65254)

    FC experiment of human PBMCs using CL594-65254

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD35 (CL594-65254, Clone:E11), or CoraLite®594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65254 targets CD35 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human CD35 from Acute monocytic leukemia cells and normal blood monocytes 相同性解析による交差性が予測される生物種
Full Name complement component (3b/4b) receptor 1 (Knops blood group)
Calculated molecular weight 224 kDa
GenBank accession numberNM_000573
Gene symbol CR1
Gene ID (NCBI) 1378
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission maxima wavelengths594 nm / 615 nm
Form Liquid
Purification MethodN/A
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.

Protocols

Product Specific Protocols
FC protocol for CL Plus 594 CD35 antibody CL594-65254Download protocol
Standard Protocols
Click here to view our Standard Protocols