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Host
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD37 Recombinant antibody, PBS Only (Detector)

CD37 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242011D6

Cat no : 84509-2-PBS

Synonyms

Tspan 26, Tetraspanin 26, Leukocyte antigen CD37, GP52 40, CD37 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD37 Recombinant antibody (84509-2-RR)

    WB analysis using 84509-2-RR (same clone as 84509-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84509-2-RR (CD37 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84509-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD37 Recombinant antibody (84509-2-RR)

    IHC staining of human tonsillitis using 84509-2-RR (same clone as 84509-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84509-2-RR (CD37 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84509-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD37 Recombinant antibody (84509-2-RR)

    IHC staining of human tonsillitis using 84509-2-RR (same clone as 84509-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84509-2-RR (CD37 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84509-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Daudi cells using CD37 Recombinant antibody (84509-2-RR)

    IF Staining of Daudi using 84509-2-RR (same clone as 84509-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD37 antibody (84509-2-RR, Clone: 242011D6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84509-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01362-1

    Cytometric bead array standard curve of MP01362-1

    Cytometric bead array standard curve of MP01362-1, CD37 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84509-3-PBS. Detection antibody: 84509-2-PBS. Standard: Eg1764. Range: 0.313-40 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84509-2-PBS targets CD37 as part of a matched antibody pair:

MP01362-1: 84509-3-PBS capture and 84509-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name CD37 molecule
Calculated molecular weight32kDa
Observed molecular weight40-52 kDa
GenBank accession numberNM_001774.3
Gene symbol CD37
Gene ID (NCBI) 951
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).