• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD37 Recombinant antibody

CD37 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242011D6

Cat no : 84509-2-RR

Synonyms

Tspan 26, Tetraspanin 26, Leukocyte antigen CD37, GP52 40, CD37 molecule

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CD37 Recombinant antibody (84509-2-RR)

    WB analysis using 84509-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84509-2-RR (CD37 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD37 Recombinant antibody (84509-2-RR)

    IHC staining of human tonsillitis using 84509-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84509-2-RR (CD37 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD37 Recombinant antibody (84509-2-RR)

    IHC staining of human tonsillitis using 84509-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84509-2-RR (CD37 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Daudi cells using CD37 Recombinant antibody (84509-2-RR)

    IF Staining of Daudi using 84509-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD37 antibody (84509-2-RR, Clone: 242011D6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inDaudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84509-2-RR targets CD37 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name CD37 molecule
Calculated molecular weight32kDa
Observed molecular weight40-52 kDa
GenBank accession numberNM_001774.3
Gene symbol CD37
Gene ID (NCBI) 951
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).

Protocols

Product Specific Protocols
WB protocol for CD37 antibody 84509-2-RRDownload protocol
IHC protocol for CD37 antibody 84509-2-RRDownload protocol
IF protocol for CD37 antibody 84509-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols