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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD4 Rabbit Recombinant Antibody

CD4 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240427E12

Cat no : 98042-1-RR

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E12

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR)

    FC experiment of human PBMCs using 98042-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR, Clone: 240427E12) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR)

    FC experiment of human PBMCs using 98042-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR, Clone: 240427E12) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98042-1-RR

    Affinity and Kinetic Characterization of 98042-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98042-1-RR against Human CD4 were performed. The affinity constant is 3.88 nM.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98042-1-RR is CD4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
FC protocol for CD4 antibody 98042-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols