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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 405 Anti-Human CD4 Rabbit Recombinant Antibody

CD4 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

240427E12

Cat no : CL405-98042

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E12

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 405 Anti-Human CD4 Rabbit Recombina (CL405-98042)

    FC experiment of human PBMCs using CL405-98042

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD3 and 5 ul CoraLite® Plus 405 Anti-Human CD4 Rabbit Recombinant Antibody (CL405-98042, Clone:240427E12)(right) or Isotype Control(left). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 405 Anti-Human CD4 Rabbit Recombina (CL405-98042)

    FC experiment of human PBMCs using CL405-98042

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD4 Rabbit Recombinant Antibody (CL405-98042, Clone:240427E12)(red), or Isotype Control(blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL405-98042 is CD4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission maxima wavelengths399 nm / 422 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
FC protocol for CL Plus 405 CD4 antibody CL405-98042Download protocol
Standard Protocols
Click here to view our Standard Protocols