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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Biotin Anti-Human CD4 (OKT4) Rabbit IgG Recombinant Antibody

CD4 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Biotin

CloneNo.

OKT4

Cat no : Biotin-65505

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4, CD4mut, CD4 molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Biotin Anti-Human CD4 (OKT4) Rabbit IgG Recombinan (Biotin-65505)

    FC experiment of human PBMCs using Biotin-65505

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD4 Rabbit IgG Recombinant Antibody (Biotin-65505, Clone: OKT4) and CoraLite®488-conjugated streptavidin, or unstained. Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Biotin Anti-Human CD4 (OKT4) Rabbit IgG Recombinan (Biotin-65505)

    FC experiment of human PBMCs using Biotin-65505

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD4 Rabbit IgG Recombinant Antibody (Biotin-65505, Clone: OKT4) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65505 targets CD4 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Human peripheral T cells 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate Biotin
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
FC protocol for Biotin CD4 antibody Biotin-65505Download protocol
Standard Protocols
Click here to view our Standard Protocols