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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5)

CD4 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

GK1.5

Cat no : CL488-65104

Synonyms

Ly 4, L3T4, GK1.5, CD4 antigen

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse splenocytes using CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5) (CL488-65104)

    IF Staining of mouse splenocytes using CL488-65104

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) at dilution of 1:500.

  • Flow cytometry (FC) experiment of mouse splenocytes using CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5) (CL488-65104)

    FC experiment of mouse splenocytes using CL488-65104

    1X10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD4 (CL488-65104, Clone:GK1.5) or 0.25 ug CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2), and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

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Tested Applications

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65104 targets CD4 in IF/ICC, FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse CTL clone V4 相同性解析による交差性が予測される生物種
Full Name CD4 antigen
GenBank accession numberBC039137
Gene symbol Cd4
Gene ID (NCBI) 12504
RRIDAB_2883178
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD4 antibody CL488-65104Download protocol
Standard Protocols
Click here to view our Standard Protocols