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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CD4 Recombinant antibody

CD4 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240427E12

Cat no : CL488-83513-7

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E12

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CoraLite® Plus 488-conjugated CD4 Recombinant anti (CL488-83513-7)

    IF Staining of THP-1 using CL488-83513-7

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 CD4 antibody (CL488-83513-7, Clone: 240427E12 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83513-7 is CD4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
Observed molecular weight55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD4 antibody CL488-83513-7Download protocol
Standard Protocols
Click here to view our Standard Protocols