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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse CD4 (RM4-5)

CD4 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

RM4-5

Cat no : CL647-65141

Synonyms

Cd4, CD4 antigen, L3T4, Ly 4

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD4 (RM4-5) (CL647-65141)

    IF Staining of mouse splenocytes using CL647-65141

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD4 (CL647-65141, Clone: RM4-5) at dilution of 1:500.

  • Flow cytometry (FC) experiment of BALB/c mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD4 (RM4-5) (CL647-65141)

    FC experiment of BALB/c mouse splenocytes using CL647-65141

    1X10^6 BALB/c mouse splenocytes were surface stained with 0.125 ug CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65141, Clone: RM4-5) (red), or 0.125 ug Control Antibody. Cells were not fixed.

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Tested Applications

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65141 targets CD4 in IF/ICC, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen BALB/c mouse thymocytes 相同性解析による交差性が予測される生物種
Full Name CD4 antigen
GenBank accession numberBC039137
Gene symbol Cd4
Gene ID (NCBI) 12504
RRIDAB_2883707
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD4 antibody CL647-65141Download protocol
Standard Protocols
Click here to view our Standard Protocols