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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human CD4 (SK3)

CD4 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

SK3

Cat no : CL647-65147

Synonyms

CD4, CD4 molecule, CD4mut, SK3

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD4 (SK3) (CL647-65147)

    FC experiment of human PBMCs using CL647-65147

    1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 405 Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD4 (CL647-65147, Clone:SK3) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD4 (SK3) (CL647-65147)

    FC experiment of human PBMCs using CL647-65147

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD4 (CL647-65147, Clone:SK3) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65147 targets CD4 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
RRIDAB_2883712
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD4 antibody CL647-65147Download protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Sunil (Verified Customer) (07-28-2023)

Used on cytotrols absolutely fine as a control conjugate for our own custom conjugates

  • Applications: Flow Cytometry
FH

Sareena (Verified Customer) (07-25-2023)

Works well as duplex stain

  • Applications: Flow Cytometry