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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APC Anti-Human CD40 (G28.5)

CD40 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

G28.5

Cat no : APC-65103

Synonyms

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using APC Anti-Human CD40 (G28.5) (APC-65103)

    FC experiment of human peripheral blood lymphocytes using APC-65103

    1X10^6 human peripheral blood lymphocytes were surface stained with 0.20 ug APC-anti-human CD40 (APC-65103, clone G28.5) (red) or 0.20 ug APC-mouse IgG1 isotype control (blue). Cells were not fixed.

  • Flow cytometry (FC) experiment of Raji cells using APC Anti-Human CD40 (G28.5) (APC-65103)

    FC experiment of Raji using APC-65103

    1X10^6 Raji cells were surface stained with 5 ul APC Anti-Human CD40 (APC-65103, Clone: G28.5) (red) or Control Antibody. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes, Raji cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65103 targets CD40 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD40 molecule, TNF receptor superfamily member 5
Calculated molecular weight 277 aa, 31 kDa
GenBank accession numberBC012419
Gene symbol CD40
Gene ID (NCBI) 958
RRIDAB_2882993
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).

Protocols

Product Specific Protocols
FC protocol for APC CD40 antibody APC-65103Download protocol
Standard Protocols
Click here to view our Standard Protocols