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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Mouse CD40 (1C10)

CD40 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C10

Cat no : CL488-65062

Synonyms

AI326936, Bp50, Cd40, CD40 antigen, GP39, HIGM1, IGM, IMD3, p50, T BAM, Tnfrsf5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of C57BL/6 mouse splenocytes using CoraLite® Plus 488 Anti-Mouse CD40 (1C10) (CL488-65062)

    FC experiment of C57BL/6 mouse splenocytes using CL488-65062

    1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 2 ug CoraLite® Plus 488 Anti-Mouse CD40 (CL488-65062, Clone:1C10) or 2 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3). Cells were not fixed.

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Tested Applications

Positive FC detected inC57BL/6 mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65062 targets CD40 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen sCD40 相同性解析による交差性が予測される生物種
Full Name CD40 antigen
GenBank accession numberBC029254
Gene symbol Cd40
Gene ID (NCBI) 21939
RRIDAB_2883157
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD40 antibody CL488-65062Download protocol
Standard Protocols
Click here to view our Standard Protocols