CoraLite®594 Anti-Human CD42b (AK2) Mouse IgG2a Recombinant Antibody
CD42b Recombinant Antibody for FC
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
FC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
AK2
Cat no : CL594-65588
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | human peripheral blood platelets |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-65588 targets CD42b in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Human platelets 相同性解析による交差性が予測される生物種 |
| Full Name | glycoprotein Ib (platelet), alpha polypeptide |
| Calculated molecular weight | 626 aa, 69 kDa |
| GenBank accession number | BC027955 |
| Gene symbol | CD42b |
| Gene ID (NCBI) | 2811 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 CD42b antibody CL594-65588 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
