CoraLite® Plus 488-conjugated CD43 Monoclonal antibody
CD43 Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2A11D6
Cat no : CL488-66224
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66224 targets CD43 in IF-P applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD43 fusion protein Ag23595 相同性解析による交差性が予測される生物種 |
Full Name | sialophorin |
Calculated molecular weight | 400 aa, 43 kDa |
Observed molecular weight | 115 kDa |
GenBank accession number | BC012350 |
Gene symbol | CD43 |
Gene ID (NCBI) | 6693 |
RRID | AB_2883288 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 CD43 antibody CL488-66224 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |