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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD44 (F10-44-2)

CD44 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human and More (1)

Applications

FC

Conjugate

Unconjugated

CloneNo.

F10-44-2

Cat no : 65063-1-Ig

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD44 (F10-44-2) (65063-1-Ig)

    FC experiment of human PBMCs using 65063-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD44 (65063-1-Ig, Clone:F10-44-2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes are gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD44 (F10-44-2) (65063-1-Ig)

    FC experiment of human PBMCs using 65063-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD44 (65063-1-Ig, Clone:F10-44-2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were not fixed. Lymphocytes are gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

65063-1-Ig targets CD44 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Purified T cells from human lymph nodes 相同性解析による交差性が予測される生物種
Full Name CD44 molecule (Indian blood group)
Calculated molecular weight 742 aa, 82 kDa
GenBank accession numberBC004372
Gene symbol CD44
Gene ID (NCBI) 960
RRIDAB_2918370
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Protocols

Product Specific Protocols
FC protocol for CD44 antibody 65063-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Exp Ther Med

Adipose-derived mesenchymal stem cell exosomes inhibit transforming growth factor-β1-induced collagen synthesis in oral mucosal fibroblasts.

Authors - Junjie Liu
View Article