FITC Plus Anti-Human CD44 (F10-44-2)
CD44 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG2a, kappa
Reactivity
Human and More (1)
Applications
IF, FC
Conjugate
FITC Plus Fluorescent Dye
CloneNo.
F10-44-2
Cat no : FITC-65063
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
| FC | See 2 publications below |
Product Information
FITC-65063 targets CD44 in IF, FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Purified T cells from human lymph nodes 相同性解析による交差性が予測される生物種 |
| Full Name | CD44 molecule (Indian blood group) |
| Calculated molecular weight | 742 aa, 82 kDa |
| GenBank accession number | BC004372 |
| Gene symbol | CD44 |
| Gene ID (NCBI) | 960 |
| RRID | AB_2883753 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 495 nm / 524 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD44 antibody FITC-65063 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Inhibition Effect of Ti3C2Tx MXene on Ice Crystals Combined with Laser-Mediated Heating Facilitates High-Performance Cryopreservation. | ||
Free Radic Biol Med Retinal cell-targeted liposomal ginsenoside Rg3 attenuates retinal ischemia-reperfusion injury via alleviating oxidative stress and promoting microglia/macrophage M2 polarization | ||
