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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 405 Anti-Human CD44 (F10-44-2) Mouse IgG2a Recombinant Antibody

CD44 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

F10-44-2

Cat no : CL405-65608

Synonyms

Epican, ECMR-III, ECMR III, CSPG8, CDW44

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 405 Anti-Human CD44 (F10-44-2) Mous (CL405-65608)

    FC experiment of human PBMCs using CL405-65608

    1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul CoraLite® Plus 405 Anti-Human CD44 (F10-44-2) Mouse IgG2a Recombinant Antibody (CL405-65608, Clone: F10-44-2) (right), or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (left). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 405 Anti-Human CD44 (F10-44-2) Mous (CL405-65608)

    FC experiment of human PBMCs using CL405-65608

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD44 (F10-44-2) Mouse IgG2a Recombinant Antibody (CL405-65608, Clone: F10-44-2) (red), or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65608 targets CD44 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD44 molecule (Indian blood group)
Calculated molecular weight 742 aa, 82 kDa
GenBank accession numberBC004372
Gene symbol CD44
Gene ID (NCBI) 960
Conjugate CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission maxima wavelengths399 nm / 422 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Protocols

Product Specific Protocols
FC protocol for CL Plus 405 CD44 antibody CL405-65608Download protocol
Standard Protocols
Click here to view our Standard Protocols