CoraLite® Plus 647 Anti-Mouse CD44 (IM7)
CD44 Monoclonal Antibody for IF/ICC, FC
Host / Isotype
Rat / IgG2b, kappa
Reactivity
Mouse
Applications
IF/ICC, FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
IM7
Cat no : CL647-65117
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | mouse splenocytes |
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-65117 targets CD44 in IF/ICC, FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Dexamethasone-induced myeloid leukemia M1 cells 相同性解析による交差性が予測される生物種 |
| Full Name | CD44 antigen |
| GenBank accession number | BC051388 |
| Gene symbol | Cd44 |
| Gene ID (NCBI) | 12505 |
| RRID | AB_2883693 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 CD44 antibody CL647-65117 | Download protocol |
| FC protocol for CL Plus 647 CD44 antibody CL647-65117 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
