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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD45 (2D1)

CD45 Monoclonal Antibody for IF-Fro, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF-Fro, FC

Conjugate

Unconjugated

CloneNo.

2D1

Cat no : 65082-1-Ig

Synonyms

L CA, GP180, EC:3.1.3.48, CD45R, B220

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using Anti-Human CD45 (2D1) (65082-1-Ig)

    IF Staining of mouse spleen using 65082-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using CD45 antibody (65082-1-Ig, Clone: 2D1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using Anti-Human CD45 (2D1) (65082-1-Ig)

    FC experiment of human peripheral blood lymphocytes using 65082-1-Ig

    1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD45 (65082-1-Ig, Clone: 2D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

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Tested Applications

Positive IF-Fro detected inmouse spleen tissue
Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-FROIF-FRO : 1:125-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65082-1-Ig targets CD45 in IF-Fro, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human PBMC 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
RRIDAB_2918385
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
IF protocol for CD45 antibody 65082-1-IgDownload protocol
FC protocol for CD45 antibody 65082-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols