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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Human CD45 (2D1)

CD45 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF, FC

Conjugate

PE Fluorescent Dye

CloneNo.

2D1

Cat no : PE-65082

Synonyms

L CA, GP180, EC:3.1.3.48, CD45R, B220

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using PE Anti-Human CD45 (2D1) (PE-65082)

    FC experiment of human peripheral blood lymphocytes using PE-65082

    1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug PE-Anti-Human CD45 (PE-65082, clone 2D1) (red) or 0.5 ug PE-mouse IgG1 isotype control (blue). Cells were not fixed.

  • Flow cytometry (FC) experiment of Raji cells using PE Anti-Human CD45 (2D1) (PE-65082)

    FC experiment of Raji using PE-65082

    1X10^6 Raji cells were surface stained with 5 ul PE Anti-Human CD45 (PE-65082, Clone:2D1) (red), or 5 ul Control Antibody. Cells were not fixed .

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Tested Applications

Positive FC detected inHuman peripheral blood lymphocytes, Raji cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

PE-65082 targets CD45 in IF, FC applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human PBMC 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
RRIDAB_2883877
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
FC protocol for PE CD45 antibody PE-65082Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Exp Ther Med

Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer

Authors - Jianping Jiang
View Article