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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Human CD45 (2D1)

CD45 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

2D1

Cat no : PE-65082

Synonyms

L CA, GP180, EC:3.1.3.48, CD45R, B220

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using PE Anti-Human CD45 (2D1) (PE-65082)

    FC experiment of human peripheral blood lymphocytes using PE-65082

    1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug PE-Anti-Human CD45 (PE-65082, clone 2D1) (red) or 0.5 ug PE-mouse IgG1 isotype control (blue). Cells were not fixed.

  • Flow cytometry (FC) experiment of Raji cells using PE Anti-Human CD45 (2D1) (PE-65082)

    FC experiment of Raji using PE-65082

    1X10^6 Raji cells were surface stained with 5 ul PE Anti-Human CD45 (PE-65082, Clone:2D1) (red), or 5 ul Control Antibody. Cells were not fixed .

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Tested Applications

Positive FC detected inHuman peripheral blood lymphocytes, Raji cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65082 targets CD45 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human PBMC 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
RRIDAB_2883877
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
FC protocol for PE CD45 antibody PE-65082Download protocol
Standard Protocols
Click here to view our Standard Protocols