Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-80297 targets CD45 in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CD45 fusion protein Ag13712 相同性解析による交差性が予測される生物種 |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| Calculated molecular weight | 1304 aa, 147 kDa |
| Observed molecular weight | 220-240 kDa |
| GenBank accession number | BC014239 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 5788 |
| ENSEMBL Gene ID | ENSG00000081237 |
| RRID | AB_3673030 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08575 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD45 antibody CL488-80297 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |