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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse CD45 (30-F11)

CD45 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

30-F11

Cat no : CL647-65087

Synonyms

Ptprc, Leukocyte common antigen, LCA, B220, 30-F11

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD45 (30-F11) (CL647-65087)

    IF Staining of mouse splenocytes using CL647-65087

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD45 (CL647-65087, Clone: 30-F11) at dilution of 1:400.

  • Flow cytometry (FC) experiment of mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD45 (30-F11) (CL647-65087)

    FC experiment of mouse splenocytes using CL647-65087

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD45 (CL647-65087, Clone: 30-F11) (red) or isotype control antibody (blue). Cells were not fixed.

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Tested Applications

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65087 targets CD45 in IF/ICC, FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse thymus or spleen 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC028512
Gene symbol CD45
Gene ID (NCBI) 19264
RRIDAB_2883673
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD45 antibody CL647-65087Download protocol
Standard Protocols
Click here to view our Standard Protocols