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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse CD45 Recombinant antibody, PBS Only

CD45 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240356G10

Cat no : 83396-5-PBS

Synonyms

CD45/PTPRC, Ptprc, Leukocyte common antigen, LCA, B220

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD45 Recombinant antibody (83396-5-RR)

    WB analysis using 83396-5-RR (same clone as 83396-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83396-5-RR (CD45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD45 Recombinant antibody (83396-5-RR)

    IHC staining of mouse spleen using 83396-5-RR (same clone as 83396-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD45 Recombinant antibody (83396-5-RR)

    IHC staining of mouse spleen using 83396-5-RR (same clone as 83396-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD45 Recombinant antibody (83396-5-RR)

    IF Staining of RAW 264.7 using 83396-5-RR (same clone as 83396-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD45 Recombinant antibody (83396-5-RR)

    IF Staining of RAW 264.7 using 83396-5-RR (same clone as 83396-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83396-5-RR

    Affinity and Kinetic Characterization of 83396-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83396-5-RR against Mouse CD45 were performed. The affinity constant is 0.415 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83396-5-PBS targets CD45 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight145 kDa
Observed molecular weight190 kDa
GenBank accession numberNM_001111316.2
Gene symbol CD45
Gene ID (NCBI) 19264
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).