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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD45 Recombinant antibody (83396-5-RR)

    WB analysis using 83396-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83396-5-RR (CD45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using CD45 Recombinant antibody (83396-5-RR)

    IHC staining of mouse colon using 83396-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using CD45 Recombinant antibody (83396-5-RR)

    IHC staining of mouse colon using 83396-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD45 Recombinant antibody (83396-5-RR)

    IHC staining of mouse spleen using 83396-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD45 Recombinant antibody (83396-5-RR)

    IHC staining of mouse spleen using 83396-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD45 Recombinant antibody (83396-5-RR)

    IF Staining of RAW 264.7 using 83396-5-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD45 Recombinant antibody (83396-5-RR)

    IF Staining of RAW 264.7 using 83396-5-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity and Kinetic Characterization of 83396-5-RR

    Affinity and Kinetic Characterization of 83396-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83396-5-RR against Mouse CD45 were performed. The affinity constant is 0.415 nM.

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CD45 Recombinant antibody

CD45 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83396-5-RR
Clone No.240356G10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ptprc, Leukocyte common antigen, L-CA, LCA, EC:3.1.3.48

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJ774A.1 cells, RAW 264.7 cells
Positive IHC detected inmouse spleen tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83396-5-RR targets CD45 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight145 kDa
Observed molecular weight190 kDa
GenBank accession numberNM_001111316.2
Gene Symbol CD45
Gene ID (NCBI) 19264
RRIDAB_3671048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP06800-4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
WB protocol for CD45 antibody 83396-5-RRDownload protocol
IHC protocol for CD45 antibody 83396-5-RRDownload protocol
IF protocol for CD45 antibody 83396-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols