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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD45 Recombinant antibody

CD45 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240356G10

Cat no : 83396-5-RR

Synonyms

B220, Cd45, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD45 Recombinant antibody (83396-5-RR)

    WB analysis using 83396-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83396-5-RR (CD45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD45/PTPRC Recombinant antibody (83396-5-RR)

    IF Staining of RAW 264.7 using 83396-5-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD45/PTPRC Recombinant antibody (83396-5-RR)

    IF Staining of RAW 264.7 using 83396-5-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

Tested Applications

Positive WB detected inJ774A.1 cells, RAW 264.7 cells
Positive IF detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83396-5-RR is CD45 Fusion Protein expressed in E. coli.

Tested Reactivity Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight145 kDa
Observed molecular weight190 kDa
GenBank accession numberNM_001111316.2
Gene symbol Ptprc
Gene ID (NCBI) 19264
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
WB protocol for CD45 antibody 83396-5-RRDownload protocol
IF protocol for CD45 antibody 83396-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols