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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 405 Anti-Human CD45RA (F8-11-13)

CD45RA Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

F8-11-13

Cat no : CL405-65226

Synonyms

CD45, B220, GP180, F8-11-13, EC:3.1.3.48

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 405 Anti-Human CD45RA (F8-11-13) (CL405-65226)

    FC experiment of human PBMCs using CL405-65226

    1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD45RO and 5 ul CoraLite® Plus 405 Anti-Human CD45RA (CL405-65226, Clone:F8-11-13) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 405 Anti-Human CD45RA (F8-11-13) (CL405-65226)

    FC experiment of human PBMCs using CL405-65226

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD45RA (CL405-65226, Clone:F8-11-13) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65226 targets CD45RA in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human T lymphocytes 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight 1304 aa, 147 kDa
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
Conjugate CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission maxima wavelengths399 nm / 422 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocols

Product Specific Protocols
FC protocol for CL Plus 405 CD45RA antibody CL405-65226Download protocol
Standard Protocols
Click here to view our Standard Protocols