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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PerCP-Cyanine5.5 Anti-Human CD45RA (HI100)

CD45RA Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Conjugate

PerCP-Cyanine5.5 Fluorescent Dye

CloneNo.

HI100

Cat no : CPY5-65108

Synonyms

CD45, B220, L CA, HI100, GP180

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using PerCP-Cyanine5.5 Anti-Human CD45RA (HI100) (CPY5-65108)

    FC experiment of human PBMCs using CPY5-65108

    1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD45RO, and 5 ul PerCP-Cyanine5.5 Anti-Human CD45RA (HI100) (CPY5-65108, Clone: HI100) or unstained. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using PerCP-Cyanine5.5 Anti-Human CD45RA (HI100) (CPY5-65108)

    FC experiment of human PBMCs using CPY5-65108

    1x10^6 human PBMCs were surface stained with 5 ul PerCP-Cyanine5.5 Anti-Human CD45RA (HI100) (CPY5-65108, Clone: HI100) (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CPY5-65108 targets CD45RA in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
Conjugate PerCP-Cyanine5.5 Fluorescent Dye
Excitation/Emission maxima wavelengths482 nm / 690nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocols

Product Specific Protocols
FC protocol for PerCP-Cyanine5.5 CD45RA antibody CPY5-65108Download protocol
Standard Protocols
Click here to view our Standard Protocols