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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APC Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody

CD45RO Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

UCHL1

Cat no : APC-65613

Synonyms

CD45, B220, L CA, GP180, EC:3.1.3.48

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using APC Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombin (APC-65613)

    FC experiment of human PBMCs using APC-65613

    1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD45RA and 5 ul APC Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody (APC-65613, Clone: UCHL1) (right), or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4) (left). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using APC Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombin (APC-65613)

    FC experiment of human PBMCs using APC-65613

    1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody (APC-65613, Clone: UCHL1) (red), or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4) (blue).Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65613 targets CD45RO in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen IL-2 dependent T cell line, CA1 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).

Protocols

Product Specific Protocols
FC protocol for APC CD45RO antibody APC-65613Download protocol
Standard Protocols
Click here to view our Standard Protocols