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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Human CD45RO (UCHL1)

CD45RO Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

UCHL1

Cat no : CL488-65150

Synonyms

CD45, B220, L CA, GP180, EC:3.1.3.48

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human peripheral blood mononuclear cells using CoraLite® Plus 488 Anti-Human CD45RO (UCHL1) (CL488-65150)

    IF Staining of human peripheral blood mononuclear cells using CL488-65150

    Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using APC Anti-Human CD45RA (APC-65108, Clone: HI100) and CoraLite® Plus 488-conjugated Anti-Human CD45RO (CL488-65150, Clone: UCHL1) at dilution of 1:300.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488 Anti-Human CD45RO (UCHL1) (CL488-65150)

    FC experiment of human PBMCs using CL488-65150

    1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD45RA and 5 ul CoraLite® Plus 488 Anti-Human CD45RO (CL488-65150, Clone:UCHL1), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488 Anti-Human CD45RO (UCHL1) (CL488-65150)

    FC experiment of human PBMCs using CL488-65150

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD45RO (CL488-65150, Clone:UCHL1), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive IF/ICC detected inhuman peripheral blood mononuclear cells
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65150 targets CD45RO in IF/ICC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen IL-2 dependent T cell line, CA1 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
RRIDAB_2883207
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD45RO antibody CL488-65150Download protocol
FC protocol for CL Plus 488 CD45RO antibody CL488-65150Download protocol
Standard Protocols
Click here to view our Standard Protocols