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CoraLite®532-conjugated CD47 Monoclonal antibody
CD47 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite®532 Fluorescent Dye
CloneNo.
1E1D8
Cat no : CL532-66304
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL532-66304 targets CD47 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD47 fusion protein Ag14132 相同性解析による交差性が予測される生物種 |
| Full Name | CD47 molecule |
| Calculated molecular weight | 323 aa, 35 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC010016 |
| Gene symbol | CD47 |
| Gene ID (NCBI) | 961 |
| Conjugate | CoraLite®532 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 537 nm / 560 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL532 CD47 antibody CL532-66304 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
