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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD5 Recombinant antibody (83543-6-RR)

    IF Staining of Jurkat using 83543-6-RR (same clone as 83543-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji and Jurkat cells using CD5 antibody (83543-6-RR, Clone: 240428A12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83543-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83543-6-RR

    Affinity and Kinetic Characterization of 83543-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83543-6-RR against Human CD5 were performed. The affinity constant is 0.747 nM.

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CD5 Recombinant antibody, PBS Only

CD5 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 83543-6-PBS
Clone No.240428A12

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Synonyms

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83543-6-PBS targets CD5 in IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD5 molecule
Calculated molecular weight55 kDa
GenBank accession numberNM_014207.4
Gene Symbol CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP06127
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.