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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD5 Recombinant antibody

CD5 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240428A12

Cat no : 83543-6-RR

Synonyms

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD5 Recombinant antibody (83543-6-RR)

    IF Staining of Jurkat using 83543-6-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji and Jurkat cells using CD5 antibody (83543-6-RR, Clone: 240428A12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity and Kinetic Characterization of 83543-6-RR

    Affinity and Kinetic Characterization of 83543-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83543-6-RR against Human CD5 were performed. The affinity constant is 0.747 nM.

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Tested Applications

Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83543-6-RR is CD5 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD5 molecule
Calculated molecular weight55 kDa
GenBank accession numberNM_014207.4
Gene symbol CD5
Gene ID (NCBI) 921
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

Protocols

Product Specific Protocols
IF protocol for CD5 antibody 83543-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols