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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD54 (15.2) Mouse IgG2a Recombinant Antibody

CD54 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

15.2

Cat no : 65567-1-MR

Synonyms

BB2, CD54, ICAM 1, ICAM1, P3.58

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD54 (15.2) Mouse IgG2a Recombinant Ant (65567-1-MR)

    FC experiment of human PBMCs using 65567-1-MR

    1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD54 (15.2) Mouse Recombinant Antibody (65567-1-MR, Clone:15.2) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Cells were then stained with APC Anti-Human CD3 (OKT3) (APC-65133, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD54 (15.2) Mouse IgG2a Recombinant Ant (65567-1-MR)

    FC experiment of human PBMCs using 65567-1-MR

    1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD54 (15.2) Mouse Recombinant Antibody (65567-1-MR, Clone: 15.2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (right), or 0.125 ug Anti-Human CD54 (ICAM-1) (15.2) (65075-1-Ig, Clone: 15.2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (left).Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65567-1-MR targets CD54 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Rheumatoid synovial cells and human monocytes 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
Calculated molecular weight 90 kDa
GenBank accession numberBC015969
Gene symbol ICAM1
Gene ID (NCBI) 3383
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Protocols

Product Specific Protocols
FC protocol for CD54 antibody 65567-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols