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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human ICAM-1/CD54 (15.2) Mouse IgG2a Recombinant Antibody, PBS Only

ICAM-1/CD54 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

15.2

Cat no : 65567-1-PBS

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM-1/CD54 (15.2) Mouse IgG2a Recombin (65567-1-MR)

    FC experiment of human PBMCs using 65567-1-MR (same clone as 65567-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD54 Mouse IgG2a Recombinant Antibody (65567-1-MR, Clone:15.2) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65567-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM-1/CD54 (15.2) Mouse IgG2a Recombin (65567-1-MR)

    FC experiment of human PBMCs using 65567-1-MR (same clone as 65567-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD54 Mouse IgG2a Recombinant Antibody (65567-1-MR, Clone:15.2) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65567-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM-1/CD54 (15.2) Mouse IgG2a Recombin (65567-1-MR)

    FC experiment of human PBMCs using 65567-1-MR (same clone as 65567-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD54 Mouse IgG2a Recombinant Antibody (65567-1-MR, Clone:15.2) (left) or 0.25 ug Anti-Human CD54 (ICAM-1) (65075-1-Ig, Clone: 15.2) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65567-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65567-1-PBS targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Rheumatoid synovial cells and human monocytes 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
Calculated molecular weight 90 kDa
GenBank accession numberBC015969
Gene symbol ICAM-1
Gene ID (NCBI) 3383
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).