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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cardinal Red™ Anti-Human ICAM-1/CD54 (15.2)

ICAM-1/CD54 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

Cardinal Red™ Fluorescent Dye

CloneNo.

15.2

Cat no : CR-65075

Synonyms

CD54 (ICAM-1), CD54, ICAM-1, ICAM1, ICAM 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Cardinal Red™ Anti-Human CD54 (ICAM-1) (15.2) (CR-65075)

    FC experiment of human PBMCs using CR-65075

    1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD54 (ICAM-1) (CR-65075, Clone:15.2) (red) or unstained. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Cardinal Red™ Anti-Human CD54 (ICAM-1) (15.2) (CR-65075)

    FC experiment of human PBMCs using CR-65075

    1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD54 (ICAM-1) (CR-65075, Clone:15.2) or unstained. Cells were not fixed. Monocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CR-65075 targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Rheumatoid synovial cells and human monocytes 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
Calculated molecular weight 90 kDa
GenBank accession numberBC015969
Gene symbol ICAM-1
Gene ID (NCBI) 3383
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission maxima wavelengths592 nm / 611 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Protocols

Product Specific Protocols
FC protocol for Cardinal Red™ ICAM-1/CD54 antibody CR-65075Download protocol
Standard Protocols
Click here to view our Standard Protocols