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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 555 Anti-Human CD55 (JS11)

CD55 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

JS11

Cat no : CL555-65271

Synonyms

CD55, CR, CROM, DAF, TC

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 555 Anti-Human CD55 (JS11) (CL555-65271)

    FC experiment of human PBMCs using CL555-65271

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD55 (CL555-65271, Clone: JS11) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 555 Anti-Human CD55 (JS11) (CL555-65271)

    FC experiment of human PBMCs using CL555-65271

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD55 (CL555-65271, Clone: JS11) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65271 targets CD55 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Calculated molecular weight 41 kDa
GenBank accession numberBC001288
Gene symbol CD55
Gene ID (NCBI) 1604
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044).

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 CD55 antibody CL555-65271Download protocol
Standard Protocols
Click here to view our Standard Protocols