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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Human CD56 (B-A19)

CD56 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

B-A19

Cat no : CL488-65264

Synonyms

CD56, MSK39, N CAM 1, NCAM, NCAM 1, NCAM1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488 Anti-Human CD56 (B-A19) (CL488-65264)

    FC experiment of human PBMCs using CL488-65264

    1X10^6 human PBMCs were surface co-stained with CL750 Anti-human CD3 and 5 ul CoraLite® Plus 488 Anti-Human CD56 (CL488-65264, Clone:B-A19), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488 Anti-Human CD56 (B-A19) (CL488-65264)

    FC experiment of human PBMCs using CL488-65264

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD56 (CL488-65264, Clone:B-A19), or Mouse IgG1 Isotype Control. Cells were not fixed. CD3-negative lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65264 targets CD56 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name neural cell adhesion molecule 1
GenBank accession numberBC014205
Gene symbol NCAM1
Gene ID (NCBI) 4684
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD56 antibody CL488-65264Download protocol
Standard Protocols
Click here to view our Standard Protocols