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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human NCAM1/CD56 Rabbit Recombinant Antibody

NCAM1/CD56 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240078A7

Cat no : CL647-98033

Synonyms

CD56, NCAM1, 240078A7, NCAM 1, NCAM

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using CoraLite® Plus 647 Anti-Human CD56 Rabbit Recombin (CL647-98033)

    FC experiment of human peripheral blood leukocytes using CL647-98033

    1x10^6 human peripheral blood leukocytes were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD56 Rabbit Recombinant Antibody (CL647-98033, Clone: 240078A7) or Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using CoraLite® Plus 647 Anti-Human CD56 Rabbit Recombin (CL647-98033)

    FC experiment of human peripheral blood leukocytes using CL647-98033

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD56 Rabbit Recombinant Antibody (CL647-98033, Clone: 240078A7) (red) or Isotype Control (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL647-98033 is NCAM1/CD56 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name neural cell adhesion molecule 1
Calculated molecular weight 95 kDa
GenBank accession numberBC047244
Gene symbol NCAM1
Gene ID (NCBI) 4684
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 NCAM1/CD56 antibody CL647-98033Download protocol
Standard Protocols
Click here to view our Standard Protocols