Validation Data Gallery
Tested Applications
| Positive FC detected in | human peripheral blood platelets |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 4 µl per 10^6 cells in a 100 µl suspension or 4 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-65185 targets CD61 in FC applications and shows reactivity with Human, Non-human primates samples.
| Tested Reactivity | Human, Non-human primates |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | N/A 相同性解析による交差性が予測される生物種 |
| Full Name | integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) |
| Calculated molecular weight | 788 aa, 87 kDa |
| GenBank accession number | BC127666 |
| Gene Symbol | Integrin beta 3 |
| Gene ID (NCBI) | 3690 |
| RRID | AB_2883819 |
| Conjugate | FITC Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 494 nm / 520 nm |
| Form | Liquid |
| Purification Method | The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| UNIPROT ID | P05106 |
| Storage Buffer | PBS with 0.1% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD61 is a 110-kDa membrane glycoprotein that is also termed integrin β3 chain or GPIIIa. CD61 can associate with integrin αV chain (CD51) or integrin αIIb chain (CD41, GPIIb) to form heterodimeric receptors for cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, plasminogen, prothrombin, thrombospondin, vitronectin and von Willebrand factor (PMID: 7694604; 9251239; 2018971; 20628538). Integrin αVβ3 (CD51-CD61) is present on endothelium, smooth muscle, osteoclasts, platelets, some fibroblasts, some activated leukocytes and tumor cells (PMID: 7694604). GPllb-llla (CD41-CD61) is restricted in distribution to platelets and cells of megakaryoblastic potential and is required for platelet aggregation (PMID: 2018971; 16322781).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC CD61 antibody FITC-65185 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |