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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 555 Anti-Human CD62L (DREG56)

CD62L Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

DREG56

Cat no : CL555-65167

Synonyms

CD62L, gp90 MEL, hLHRc, L selectin, LAM 1, LAM1, LECAM1, Leu 8, Leukocyte adhesion molecule 1, LNHR, LSEL, Lyam 1, LYAM1, Lymph node homing receptor, PLNHR, selectin L, SELL, TQ1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 555 Anti-Human CD62L (DREG56) (CL555-65167)

    FC experiment of human PBMCs using CL555-65167

    1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 555 Anti-Human CD62L (CL555-65167, Clone: DREG56) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 555 Anti-Human CD62L (DREG56) (CL555-65167)

    FC experiment of human PBMCs using CL555-65167

    1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 555 Anti-Human CD62L (CL555-65167, Clone: DREG56) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65167 targets CD62L in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen PMA-activated human peripheral blood leukocytes 相同性解析による交差性が予測される生物種
Full Name selectin L
Calculated molecular weight 42 kDa
GenBank accession numberBC020758
Gene symbol CD62L
Gene ID (NCBI) 6402
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 CD62L antibody CL555-65167Download protocol
Standard Protocols
Click here to view our Standard Protocols